Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human GR around the phosphorylation site of serine 226/234/246 (L-L-S-P-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P04150
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:10000
Alter Name:
GCR, GRL, GRL1, NR3C1, glucocorticoid receptor
Specificity Sensitivity:
GR (Ab-226) antibody detects endogenous levels of total GR protein.
References:
Kyung Jin Kwak, J. Exp. Bot., Nov 2005; 56: 3007 - 3016.
Maite de Llanos, Hum. Reprod., Jan 2005; 20: 216 - 220.
M. Lynch, Mol. Hum. Reprod., Jul 2005; 11: 507 - 512.
Valeriya P. Makarenkova, J. Immunol., Feb 2006; 176: 2085 - 2094.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human brain tissue using GR (Ab-226) antibody (#B0432).
Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 15mins), using GR (Ab-226) antibody (#B0432).