Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Fyn around the phosphorylation site of tyrosine 530 (P-Q-YP-Q-P).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P06241
Applications:
WB: 1:500~1:1000 ELISA: 1:1000
Other Names:
EC 2.7.10.2; FYN; kinase Fyn; P59-FYN; SLK; SYN
Specificity Sensitivity:
Fyn (Ab-530) antibody detects endogenous levels of total Fyn protein.
References:
Kawakami T., Mol. Cell. Biol. 6:4195-4201(1986).
Semba K., Proc. Natl. Acad. Sci. U.S.A. 83:5459-5463(1986).
Rigley K., J. Immunol. 154:1136-1145(1995).
Western blot analysis of extracts from HUVEC cells, MCF-7 cells and 293 cells, using Fyn (Ab-530) antibody.