• Catalog Number:

  E1A0417B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Cyclin D1 around the phosphorylation site of threonine 286 (A-C-TP-P-T).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P24385

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

• Other Names:

  BCL-1 oncogene, BCL1, CCND1, CYL-1, G1/S-specific cyclin D1, PRAD1, PRAD1 oncogene

• Specificity Sensitivity:

  Cyclin D1 (Ab-286) antibody detects endogenous levels of total Cyclin D1 protein.

• References:

  Zhiping Li, Mol. Cell. Biol., Jun 2006; 26: 4240 - 4256. Toshiyuki Sakamaki, Mol. Cell. Biol., Jul 2006; 26: 5449 - 5469. Yuki Ishii, J Natl Cancer Inst, Sep 2006; 98: 1238 - 1247.

Peptide - +

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Cyclin D1 (Ab-286) antibody. Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 30mins), using Cyclin D1 (Ab-286) antibody.