Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Chk2 around the phosphorylation site of threonine 387 (C-G-T-P-T).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
O96017
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:5000
Alter Name:
CHEK2, CHK2, Cds1, Chk2, RAD53, kinase Chk2
Specificity Sensitivity:
Chk2 (Ab-387) antibody detects endogenous levels of total Chk2 protein.
References:
Atsushi Hirao, Mol. Cell. Biol., Sep 2002; 22: 6521 - 6532.
Julie K. Schwarz, Mol. Cancer Res., Jun 2003; 1: 598.
Shutong Yang, J. Biol. Chem., Sep 2006; 281: 26645 - 26654.
Xingzhi Xu, Mol. Cell. Biol., Jun 2002; 22: 4419 - 4432.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk2 (Ab-387) Antibody (#B0414).
Western blot analysis of extracts from Jurkat cells, using Chk2 (Ab-387) Antibody (#B0414).