Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human CDC6 around the phosphorylation site of serine 54 (P-L-S-P-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
Q99741
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:5000
Alter Name:
CDC18, CDC6 cell division cycle 6 homolog, CDC6-related protein
Specificity Sensitivity:
CDC6 (Ab-54) antibody detects endogenous levels of total CDC6 protein.
References:
Jonathan R. Hall, Mol. Biol. Cell, Jun 2007; 10.1091.
Luo Feng, Mol. Biol. Cell, May 2000; 11: 1673.
Arturo Calzada, J. Biol. Chem., Mar 2000; 275: 9734.
Rajesh Kasiviswanathan, Nucleic Acids Res., Sep 2005; 33: 4940 - 4950.
Peptide - +
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using CDC6 (Ab-54) antibody (#B0413).
Western blot analysis of extracts from HT-29 cells, using CDC6 (Ab-54) antibody (#B0413).