• Catalog Number:

  E1A0406B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of threonine 450(T-I-TP-P-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P31749

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000

• Other Names:

  AKT, AKT1 kinase, C-AKT, PKB, PKB-alpha, Protein kinase B, RAC, RAC-PK-alpha, RAC-alpha serine/threonine kinase, kinase Akt1

• Specificity Sensitivity:

  Akt (Ab-450) antibody detects endogenous levels of total Akt protein.

• References:

  Payaningal R. Somanath, J. Biol. Chem., Jun 2007; 10.1074. Xiaoming Ju, PNAS, May 2007; 104: 7438 - 7443. Brian DeBosch, Circulation, May 2006; 113: 2097 - 2104.

Peptide - +

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Akt (Ab-450) antibody. Western blot analysis of extracts from NIH/3T3 cells, treated with Serum (10%, 30mins), using Akt (Ab-450) antibody.