Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human SGK around the phosphorylation site of serine 422 (G-F-SP-Y-A).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
O00141
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
SGK1, SERUM/GLUCOCORTICOID-REGULATED KINASE
Specificity Sensitivity:
SGK (Ab-422) antibody detects endogenous levels of total SGK protein.
References:
Nicola Perrotti, J. Biol. Chem., Mar 2001; 276: 9406 - 9412.
Yun L. Ma, Learn. Mem., Mar 2006; 13: 114 - 118.
Ge-Xin Wang, Am J Physiol Heart Circ Physiol, Aug 2004; 287: H533 - H544.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SGK (Ab-422) antibody.
Western blot analysis of extracts from Jurkat cells, using SGK (Ab-422) antibody.