• Catalog Number:

  E1A0031B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from humanSmad3 around the phosphorylation site of serine 425 (C-S-S-V-SP).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P84022

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Other Names:

  JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3

• Specificity Sensitivity:

  Smad3 (Ab-425) antibody detects endogenous levels of total Smad3 protein.

• References:

  Baljit Walia, FASEB J, Sep 2003; 10.1096/fj.02-1211fje. Evangelia Pardali, J. Biol. Chem., Feb 2000; 275: 3552. HUGO CEULEMANS, Physiol Rev, Jan 2004; 84: 1.

Peptide - +

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Smad3 (Ab-425) antibody. Western blot analysis of extracts from 293 cells, treated with UV (5mins), Smad3 (Ab-425) antibody.