• Catalog Number:

  E1A0030B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized phosphopeptide derived from human Smad2 around the phosphorylation site of serine 467 (C-S-S-M-SP).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q15796

• Applications:

  WB: 1:300~1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

• Alter Name:

  JV18-1, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2, Mothers against decapentaplegic homolog 2, Smad 2

• Specificity Sensitivity:

  Smad2 (Phospho-Ser467) antibody detects endogenous levels of Smad2 only when phosphorylated at serine 467.

• References:

  Kamaraju AK, J Biol Chem 2005 Jan 14; 280(2): 1024-36. Ottesen JJ, Biochemistry 2004 May 18; 43(19): 5698-706. Bourguignon LY, J Biol Chem 2002 Oct 18; 277(42): 39703-12. Souchelnytskyi S, J Biol Chem 1997 Oct; 272(44): 28107-15.

• All Sites:

  Human: Ser467; Mouse: Ser467; Rat: Ser467

Western blot analysis of extracts from HeLa cells treated with PMA (125ng/ml, 15mins), using Smad2 (Phospho-Ser467) antibody (#A0030).