• Catalog Number:

  E1A8541B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human ROCK2 around the phosphorylation site of tyrosine 722 (K-I-YP-E-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O75116

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000

• Other Names:

  KIAA0619; kinase ROCK2; p164 ROCK-2; Rho-associated coiled-coil containing protein kinase 2; Rho-associated protein kinase 2; RhoA-binding serine/threonine kinase alpha; ROK-alpha

• Specificity Sensitivity:

  ROCK2 (Ab-722) antibody detects endogenous levels of total ROCK2 protein.

• References:

  Wilson R.K., Nature 434:724-731(2005). Gygi S.P., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). Mann M., Cell 127:635-648(2006).

Western blot analysis of extracts from Cos7 cells, using ROCK2 (Ab-722) antibody.