• Catalog Number:

  E1A8439B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human TOP2A around the phosphorylation site of serine 1525 (L-E-E-SP-D).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P11388

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Other Names:

  DNA topoisomerase 2-alpha; EC=5.99.1.3; TOP2

• Specificity Sensitivity:

  TOP2A (Ab-1525) antibody detects endogenous levels of total TOP2A protein.

• References:

  Tsai-Pflugfelder M., Proc. Natl. Acad. Sci. U.S.A. 85:7177-7181(1988). Wasserman R.A., Cancer Res. 53:3591-3596(1993). Lang A.J., Gene 221:255-266(1998). ------------------------------------------------------------------------------------------------------------------------------------------

Western blot analysis of extracts from 293 cells and K562 cells, using TOP2A (Ab-1525) antibody.