• Catalog Number:

  E1A8340B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human eEF2K around the phosphorylation site of serine 359 (G-SP-P-R-V).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 87%, Positives = 100%);Rat (Identities = 87%, Positives = 100%)

• SwissProtNo:

  O00418

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  Calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase; EC 2.7.11.20; eEF-2 kinase; eEF-2K; EF2K; Elongation factor 2 kinase; kinase Eef2k

• Specificity Sensitivity:

  eEF2K (Ab-359) antibody detects endogenous levels of total eEF2K protein.

• References:

  Ryazanov A.G., Proc. Natl. Acad. Sci. U.S.A. 94:4884-4889(1997). Brill L.M., Anal. Chem. 76:2763-2772(2004). Olsen J.V., Cell 127:635-648(2006). ------------------------------------------------------------------------------------------------------------------------------------------

Western blot analysis of extracts from Jurkat cells, using eEF2K (Ab-359) antibody.