Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from Internal of human 4E-BP1.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human (Identities = 100%, Positives = 100%)
SwissProtNo:
Q13541
Applications:
IHC: 1:50~1:100 ELISA: 1:40000
Other Names:
4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6; PHAS-1; PHAS-I
Specificity Sensitivity:
4E-BP1 antibody detects endogenous levels of total 4E-BP1 protein.
References:
Pause A., Nature 371:762-767(1994).
Gevaert K., Nat. Biotechnol. 21:566-569(2003).
Haghighat A., EMBO J. 14:5701-5709(1995).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 antibody.