Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Estrogen Receptor-β around the phosphorylation site of serine 87 (H-L-SP-P-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human (Identities = 100%, Positives = 100%)
SwissProtNo:
Q92731
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Other Names:
ESR2; ESTRB; Estrogen receptor beta; NR3A2
Specificity Sensitivity:
Estrogen Receptor-β (Ab-87) antibody detects endogenous levels of total Estrogen Receptor-β protein.
References:
Ogawa S., Biochem. Biophys. Res. Commun. 243:122-126(1998).
Moore J.T., Biochem. Biophys. Res. Commun. 247:75-78(1998).
Mosselman S., FEBS Lett. 392:49-53(1996).
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Western blot analysis of extracts from MCF-7 cells, using Estrogen Receptor-β (Ab-87) antibody.