Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized peptide derived from Internal of human GRK2.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human (Identities = 100%, Positives = 100%);Mouse (Identities = 91%, Positives = 100%);
Rat (Identities = 91%, Positives = 100%)
SwissProtNo:
P25098
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
Other Names:
ADRBK1; ARBK1; BARK; BARK1; beta-adrenergic receptor kinase 1; beta-ARK-1; EC 2.7.11.15; G protein receptor kinase 2; G- protein coupled receptor kinase 2; kinase GRK2
Specificity Sensitivity:
GRK2 (Ab-685) antibody detects endogenous levels of total GRK2 protein.
References:
Benovic J.L., FEBS Lett. 283:122-126(1991).
Chuang T.T. , J. Biol. Chem. 267:6886-6892(1992).
Penn R.B., J. Biol. Chem. 269:14924-14930(1994).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using GRK2 (Ab-685) antibody.
Western blot analysis of extracts from Jurkat cells, using GRK2 (Ab-685) antibody.