Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human SLP-76 around the phosphorylation site of tyrosine 128 (G-D-YP-E-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
LCP2; Lymphocyte cytosolic protein 2; SH2 domain-containing leucocyte protein of 76 kDa; SLP-76 tyrosine phosphoprotein
Specificity Sensitivity:
SLP-76 (Ab-128) antibody detects endogenous levels of total SLP-76 protein.
References:
Jackman J.K., J. Biol. Chem. 270:7029-7032(1995).
Moog-Lutz C., J. Biol. Chem. 276-22375-22381(2001).
Lindholm C.K., Eur. J. Biochem. 269:3279-3288(2002).
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Peptide - +
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using SLP-76 (Ab-128) antibody.
Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 5mins), using SLP-76 (Ab-128) antibody.