• Catalog Number:

  E1A8199B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human WEE1 around the phosphorylation site of serine 53 (E-D-SP-A-F).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  P30291

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  EC 2.7.10.2; kinase WEE1; Wee1-like protein kinase; WEE1A; WEE1hu

• Specificity Sensitivity:

  WEE1 (Ab-53) antibody detects endogenous levels of total WEE1 protein.

• References:

  Cichutek A.,Cytogenet. Cell Genet. 93:277-283(2001). Lu R.,J. Biol. Chem. 279:31164-31170(2004). Beausoleil S.A.,Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). ------------------------------------------------------------------------------------------------------------------------------------------

Western blot analysis of extracts from 293 cells, using WEE1 (Ab-53) antibody.