• Catalog Number:

  E1A8185B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human SIK around the phosphorylation site of threonine 182 (L-S-TP-W-C).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%);Rat (Identities = 92%, Positives = 92%)

• SwissProtNo:

  P57059

• Applications:

  WB: 1:500~1:1000 ELISA: 1:40000

• Other Names:

  EC 2.7.11.1; salt-inducible kinase; serine/threonine-protein kinase SNF1-like kinase 1; serine/threonine-protein kinase SNF1LK; SN1L1; SNF1-like kinase; SNF1LK

• Specificity Sensitivity:

  SIK (Ab-182) antibody detects endogenous levels of total SIK protein.

• References:

  Lizcano J.M., EMBO J. 23:833-843(2004). Hattori M., Nature 405:311-319(2000). Ota T., Nat. Genet. 36:40-45(2004). ------------------------------------------------------------------------------------------------------------------------------------------

Western blot analysis of extracts from COLO cells, treated with PMA (125ng/ml, 30mins), using SIK (Ab-182) antibody.