• Catalog Number:

  E1A8141B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human MAP2K7 around the phosphorylation site of threonine 275 (A-K-TP-R-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%)

• SwissProtNo:

  O43318

• Applications:

  WB: 1:500~1:2000 ELISA: 1:20000

• Other Names:

  EC 2.7.11.25; kinase TAK1; M3K7; MAP3K7; Mitogen-activated protein kinase kinase kinase 7; TGF-beta- activated kinase 1; Transforming growth factor-beta-activated kinase 1

• Specificity Sensitivity:

  MAP2K7 (Ab-275) antibody detects endogenous levels of total MAP2K7 protein.

• References:

  Sakurai H., Biochem. Biophys. Res. Commun. 243:545-549(1998). Mungall A.J., Nature 425:805-811(2003). The MGC Project Team; Genome Res. 14:2121-2127(2004).

Western blot analysis of extracts from 293 cells, treated with insulin (0.01U/ml, 15mins), using MAP2K7 (Ab-275) antibody. Western blot analysis of extracts from A549 cells and JurKat cells, using MAP2K7 (Ab-275) antibody.