Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Tau around the phosphorylation site of Ser235.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P10636
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Specificity Sensitivity:
Tau (Ab-235) Antibody detects endogenous levels of Tau protein around Serine 235.
References:
Alonso Adel C, et al. (2004) J Biol Chem. 279(33): 34873-34881.
Liu F, et al. (2002) FEBS Lett. 530(1-3): 209-214.
Sengupta A, et al. (1998) Arch Biochem Biophys. 357(2): 299-309.
Western blot analysis of extracts using Tau (Ab-235) Antibody (#B7238). Line1: K562 cells; Line2: COLO205 cells.