Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human α-Synuclein around the phosphorylation site of tyrosine 133 (E-G-Y-Q-D).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P37840
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Alter Name:
NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, SYN, SYUA, alpha-synuclein, synuclein alpha
Specificity Sensitivity:
α-Synuclein (Ab-133) antibody detects endogenous levels of total α-Synuclein protein.
References:
Yangfu Jiang, Cancer Res., Jul 2004; 64: 4539 - 4546.
Alessandro Negro, FASEB J, Dec 2001; 10.1096.
ALESSANDRA RECCHIA, FASEB J, Apr 2004; 18: 617 - 626.
Christopher E. Ellis, J. Biol. Chem., Feb 2001; 276: 3879 - 3884.
Western blot analysis of extracts from 293 cells, treated with Etoposide (25μM, 60mins), using α-Synuclein (Ab-133) antibody (#B7235, Line 1 and 2).