Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human synapsin around the phosphorylation site of serine 9 (R-L-SP-D-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P17600
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Alter Name:
SYN1, Synapsin-1
Specificity Sensitivity:
synapsin (Ab-9) antibody detects endogenous levels of total synapsin protein. The antibody is expected to recognize phosphorylated serine 9 of all synapsin isoforms.
References:
Diviya Sinha, et,al. (2005) Am J Physiol Renal Physiol; 288: F703-F713.
Franco Onofri, et,al. (2000) J. Biol. Chem; 275: 29857.
Dario Bonanomi, et,al. (2005) J. Neurosci; 25: 7299-7308.
Hiroshi Tokumitsu, et,al. (2005) J. Biol. Chem; 280: 35108-35118.
Western blot analysis of extracts from 293 cells untreated or treated with PMA (200nM, 30min), using synapsin (Ab-9) antibody (Line 1 and 2) and synapsin (phospho-Ser9) antibody (Line 3 and 4).