Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human S6 Ribosomal protein around the phosphorylation site of serine 240 or serine 244 (S-T-S-K-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P62753
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Alter Name:
40S ribosomal protein S6, Phosphoprotein NP33, RPS6, RS6, ribosomal protein S6
Specificity Sensitivity:
S6 Ribosomal protein (Ab-240/244) antibody detects endogenous levels of total S6 Ribosomal protein.
References:
McBride K, et al. (1998) Mol Cell Biol 18(9): 5073-5081.
Williams AJ, et al. (2003) Plant Physiol 132(4): 2086-2097.
Wilson MA, et al. (1997) Biochem J 325(Pt 1): 217-222.
Arnesen T, et al. (2005) Biochem J 386(Pt 3): 433-443.
Western blot analysis of extracts from 293 cells treated with serum (10%, 15mins), using S6 Ribosomal protein (Ab-240/244) antibody (#B7214).