• Catalog Number:

  E1A7195B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O15530

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Specificity Sensitivity:

  PDK1 (Ab-241) antibody detects endogenous levels of total PDK1 protein.

• References:

  Scheid MP,et al. (2005)Mol Cell Biol; 25(6): 2347-63 Chen H, et al. (2001) Biochemistry; 40(39): 11851-9 Sato S,et al. (2002) J Biol Chem; 277(42): 39360-7 Lim MA, et al.(2003)Proc Natl Acad Sci U S A; 100(24): 14006-11 Beausoleil SA, et al. (2004)Proc Natl Acad Sci U S A; 101(33): 12130-5

Peptide - + Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using PDK1 (Ab-241) antibody (#B7195).

Western blot analysis of extracts from MDA-MB-435 cells using PDK1 (Ab-241) antibody (#B7195).