Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 9 (D-P-SP-V-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P04637
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Specificity Sensitivity:
p53 (Ab-9) antibody detects endogenous levels of total p53 protein.
References:
Eric Meyer, et al. (2005) Cancer Res. 65: 4261-4272.
Thomas Buschmann, et al. (2000) Cancer Res. 60: 896-900.
Gregory A. Mayr, et al. (1995) Cancer Res. 55: 2410-2417.
Simon J. Allison et al. (2003) Cancer Res. 63: 6674-6679.
Western blot analysis of extracts from COS cells untreated or treated with UV, using p53 (Ab-9) antibody (#B7186, Line1 and 2).