Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 6.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P04637
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Specificity Sensitivity:
p53 (Ab-6) antibody detects endogenous levels of total p53 protein.
References:
Thompson T, et al. (2004) J Biol Chem; 279(51): 53015-22.
Jackson MW, et al. (2004) Oncogene.
Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385.
Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.
Lohrum, M. and Scheidtmann, K.H. (1996) Oncogene 13, 2527-2539.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Ab-6) antibody (#B7185).
Western blot analysis of extracts using p53 (Ab-6) antibody (#B7185).