Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of Serine 37.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P04637
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Specificity Sensitivity:
p53 (Ab-37) Antibody detects endogenous levels of p53 protein around Serine 37.
References:
Dhavan, R. and Tsai, L.H. (2001) Nat Rev Mol Cell Biol. 2: 749-759.
Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064.
Mayo LD, et al.(2005) J Biol Chem. 280(28):25953-25959.
Thompson T, et al.(2004) J Biol Chem. 279(51): 53015-53022.
Jackson MW, et al.(2004) Oncogene. 23(25):4477-4487.
Western blot analysis of extracts from K562 cells. Line1: Using p53 (Ab-37) Antibody; Line2: Using the same antibody preincubated with synthesized peptide.