• Catalog Number:

  E1A7181B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human p53 around the phosphorylation site of serine 315.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  P04637

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Specificity Sensitivity:

  p53 (Ab-315) antibody detects endogenous levels of total p53 protein.

• References:

  Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934. Lohrum, M. et.al. (1996) Oncogene 13, 2527-2539. Pospísilová S, et al. (2004) Biochem J; 378(Pt 3): 939-47. Merrick BA, et al. (2001) Biochemistry; 40(13): 4053-66.

Western blot analysis using p53 (Ab-315) antibody (#B7181). Line1: HT29 cells; Line 2: MDA-MB-435 cells; Line 3: K562 cells; Line 4: COLO205 cells.