• Catalog Number:

  E1A7175B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human p21Cip1 around the phosphorylation site of Threonine 145.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  P38936

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Specificity Sensitivity:

  p21Cip1 (Ab-145) Antibody detects endogenous levels of p21Cip1 protein around Threonine 145.

• References:

  Rössig L, et al. (2002) J Biol Chem 277(12): 9684-9689. Rössig L, et al. (2001) Mol Cell Biol 21(16): 5644-5657. Zhou BP, et al. (2001) Nat Cell Biol 3(3): 245-252.

Western blot analysis of extracts from HeLa cells treated with EGF. Line1: Using p21Cip1 (Ab-145) Antibody (#B7175); Line2: Using the same antibody preincubated with synthesized peptide.