• Catalog Number:

  E1A7172B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human NF-κB p65 around the phosphorylation site of threonine 254.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q04206

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  NF-κB p65 (Ab-254) antibody detects endogenous levels of total NF-κB p65 protein.

• References:

  Yeh PY, et al. (2004) J Biol Chem. 279(25): 26143-26148. Ryo A, et al. (2003) Mol Cell.12 (6): 1413-1426. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p65 (Ab-254) antibody (#B7172).

Western blot analysis of extracts from Raw264.7 cells using NF-κB p65 (Ab-254) antibody (#B7172).