Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human NF-κB p105/p50 around the phosphorylation site of serine 893.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P19838
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Specificity Sensitivity:
NF-κB p105/p50 (Ab-893) antibody detects endogenous levels of NF-κB p105/p50 protein around serine 893.
References:
Hou S, et al. (2003) J Biol Chem. 278(46): 45994-45998.
Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179.
Baeuerle P A, et al. (1996) Cell 87:13-20.
Haskill S, et al. (1991) Cell 65:1281-1289.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF-κB p105/p50 (Ab-893) antibody (#B7167).
Western blot analysis of extracts from HeLa cells using NF-κB p105/p50 (Ab-893) antibody (#B7167).