Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Myc around the phosphorylation site of threonine 358.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P01106
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Specificity Sensitivity:
Myc (Ab-358) antibody detects endogenous levels of total Myc protein.
References:
Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702.
Blackwood E M, et al. (1991) Science. 251:1211-1217.
Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.
Grandori C, et al. (2000) Annu Rev Cell Dev Biol. 16: 653-699.
Peptide - +
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using Myc (Ab-358) antibody.
Western blot analysis of extracts from NIH/3T3 cells using Myc (Ab-358) antibody.