Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Met around the phosphorylation site of tyrosine 1349 (E-H-YP-V-H).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P08581
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Alter Name:
HGF receptor, HGF-SF receptor, Hepatocyte growth factor receptor precursor, Met proto- oncogene tyrosine kinase, c-met, kinase Met
Specificity Sensitivity:
Met (Ab-1349) antibody detects endogenous levels of total Met protein. This antibody may cross-react with other activated protein tyrosine kinases.
References:
Fan S, et al. (2001) Mol Cell Biol; 21(15): 4968-4984.
Schiering N, et al. (2003) Proc Natl Acad Sci U S A; 100(22): 12654-12659.
Plopper GE, et al. (1995) Mol Biol Cell; 6(10): 1349-1365.
Ponzetto C, et al. (1993) Mol Cell Biol; 13(8): 4600-4608.
Jackson PA, et al. (2001) Plant Physiol; 127(3): 1065-1076.
Western blot analysis of extracts from HepG2 cells, using Met (Ab-1349) antibody (Line1 and 2) and Met (phospho-Tyr1349) antibody (Line 3 and 4).