Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Met around the phosphorylation site of tyrosine 1234 (K-E-YP-Y-S).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P08581
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Alter Name:
MET Hepatocyte growth factor receptor [Precursor]
Specificity Sensitivity:
Met (Ab-1234) antibody detects endogenous levels of total Met protein.
References:
Gherardi E. et al. (2003).Proc Natl Acad Sci U S A. 100(21): 12039-12044.
Shiu SH. et al. (2001) Proc Natl Acad Sci U S A. 98(19): 10763-10768.
Hughes AL. et al. (2001) Genome Res. 11(5): 771-780.
Onuchic LF. et al. (2002) Am J Hum Genet. 70(5): 1305-1317.
Western blot analysis of extracts from HepG2 cells using Met (Ab-1234) antibody (Line 1 and 2) and Met (phospho- Tyr1234) antibody (Line 3 and 4).