• Catalog Number:

  E1A7146B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human MEK1 around the phosphorylation site of serine 217 (I-D-SP-M-A).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  Q02750

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK1, MP2K1, PRKMK1, kinase MEK1

• Specificity Sensitivity:

  MEK1 (Ab-217) antibody detects endogenous levels of total MEK1 protein.

• References:

  Crews, C.M. et al. (1992) Science 258, 478-480. Alessi, D.R. et al. (1994) EMBO J. 13, 1610-1619. Rosen, L.B. et al. (1994) Neuron 12, 1207-1221. Cowley, S. et al. (1994) Cell 77, 841-852.

Western blot analysis of extracts from Hela cells untreated or treated with PMA, using MEK1 (Ab-217) antibody (#B7146, Line1 and 2).