• Catalog Number:

  E1A7116B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human IκB-α around the phosphorylation site of tyrosine 42 (E-E-YP-E-Q).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P25963

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

• Alter Name:

  I-kappa-B-alpha, IKBA, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappaB inhibitor alpha, NFKBI, NFKBIA, RL/IF-1

• Specificity Sensitivity:

  IκB-α (Ab-42) antibody detects endogenous levels of total IκB-α protein.

• References:

  Béraud C, et al. (1999) Proc Natl Acad Sci U S A 96(2): 429-434. Sundström S, et al. (2005) J Virol 79(4): 2230-2239. Liu L, et al. (1998) Mol Cell Biol 18(7): 4221-4234. Shrivastava A, et al. (1998) J Virol 72(12): 9722-9728. Franzoso G, et al. (1997) Genes Dev 11(24): 3482-3496.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IκB-α (Ab-42) antibody (#B7116).

Western blot analysis of extracts from 293 cells untreated or treated with TNF-α (20ng/ml, 30min), using IκB-α (Ab-42) antibody (#B7116).