Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human ICAM-1 around the phosphorylation site of tyrosine 512(K-K-YP-R-L).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P05362
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Other Names:
CD54; ICA1; ICAM1; intercellular adhesion molecule-1 precursor; major group rhinovirus receptor
Specificity Sensitivity:
ICAM-1 (Ab-512) antibody detects endogenous levels of total ICAM-1 protein.
References:
Simmons D., Nature 331:624-627(1988).
Staunton D.E., Cell 52:925-933(1988).
Tomassini J.E., Proc. Natl. Acad. Sci. U.S.A. 86:4907-4911(1989).
Western blot analysis of extracts from HepG2 cells, using ICAM-1 (Ab-512) antibody.