Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 925.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q05397
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Specificity Sensitivity:
FAK (Ab-925) antibody detects endogenous levels of total FAK protein.
References:
Sanders MA, et al. (2005) J Biol Chem; 280(25): 23516-22.
Cherubini A, et al. (2005) Mol Biol Cell; 16(6): 2972-83.
Toriumi Y, et al. (2003) FEBS Lett; 553(3): 419-22.
Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FAK (Ab-925) antibody (#B7083).
Western blot analysis of extracts from HeLa cells using FAK (Ab-925) antibody (#B7083).