Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human FAK around the phosphorylation site of tyrosine 861.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
Q05397
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Specificity Sensitivity:
FAK (Ab-861) antibody detects endogenous levels of total FAK protein.
References:
Shi Q, et al. (2003) Mol Biol Cell; 14(10): 4306-15.
Vadlamudi RK, et al. (2003) FEBS Lett; 543(1-3): 76-80.
Eliceiri BP, et al. (2002) J Cell Biol Apr 01; 157(1): 149-60.
Abu-Ghazaleh R, (2001) et al. Biochem J; 360(Pt 1): 255-64.
Slack JK, et al.(2001) Oncogene; 20(10): 1152-63.
| HeLa | | HepG2 | HeLa | 293
Western blot analysis using FAK (Ab-861) antibody (#B7082, Line 1 and 2) and FAK (phospho-Tyr861) antibody (#11059, Line 3, 4, 5 and 6).