Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 566 (Y-K-TP-L-R).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P15311
Applications:
WB: 1:500~1:1000 ELISA: 1:20000
Alter Name:
Cytovillin, EZRI, VIL2, Villin 2, p81
Specificity Sensitivity:
Ezrin (Ab-566) antibody detects endogenous levels of total Ezrin protein.
References:
Zhou R, et al. (2005) J Cell Sci: 118 (Pt 19): 4381-91.
Shiue H, et al.(2005) J Biol Chem: 280(2): 1688-95.
Zhao H, et al. (2004) Proc Natl Acad Sci U S A: 101(25): 9485-90.
Fievet BT, et al. (2004) J Cell Biol: 164(5): 653-9.
Western blot analysis of extracts from A431 cells, untreated or EGF-treated (200ng/ml, 30min) using Ezrin (Ab-566) antibody (Line 1 and 2) and Ezrin (phospho-Tyr566) antibody (Line 3 and 4).