Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Estrogen Receptor-α around the phosphorylation site of serine167.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human
SwissProtNo:
P03372
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Specificity Sensitivity:
Estrogen Receptor-α (Ab-167) antibody detects endogenous levels of total Estrogen Receptor-α protein.
References:
Shah YM, et al. (2005). Mol Endocrinol.19 (3): 732-748.
Sun M, et al. (2001). Cancer Res.61 (16): 5985-5991.
Campbell RA, et al. (2001). J Biol Chem.276 (13): 9817-9824.
Chen D, et al. (2000). Mol Cell.6 (1): 127-137.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Ab-167) antibody (#B7078).
Western blot analysis of extracts from MCF7 cells using Estrogen Receptor-α (Ab-167) antibody (#B7078).