• Catalog Number:

  E1A7076B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Estrogen Receptor-α around the phosphorylation site of serine 104/106 (V-SP-P-SP-P).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse

• SwissProtNo:

  P03372

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Alter Name:

  ER, ESR, ESR1, ESTR, ESTRA, Estradiol receptor, Estrogen receptor, NR3A1

• Specificity Sensitivity:

  Estrogen Receptor-α (Ab-104/106) antibody detects endogenous levels of total Estrogen Receptor-α protein.

• References:

  Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014. Dutertre M, et al. (2003). Mol Endocrinol.17 (7): 1296-1314. Chen D, et al. (2000). Mol Cell.6 (1): 127-137.

Peptide - + Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Ab-104/106) antibody (#B7076).

Western blot analysis of extracts from MCF7 cells, untreated or treated with Estradio, using Estrogen Receptor-α (Ab-104/106) antibody (Line 1 and 2) and Estrogen Receptor-α (phospho-Ser104/106) antibody ( Line 3 and 4).