Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Estrogen Receptor-α around the phosphorylation site of serine 104.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P03372
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100
ELISA: 1:20000
Specificity Sensitivity:
Estrogen Receptor-α (Ab-104) antibody detects endogenous levels of total Estrogen Receptor-α protein.
References:
Medunjanin S, et al. (2005). J Biol Chem.80 (38):33006-33014.
Dutertre M, et al. (2003). Mol Endocrinol.17 (7): 1296-1314.
Chen D, et al. (2000). Mol Cell.6 (1): 127-137.
Rogatsky I, et al. (1999). J Biol Chem.274 (32): 22296-22302.
Peptide - +
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Estrogen Receptor-α (Ab-104) antibody.
Western blot analysis of extracts from 293 cells using Estrogen Receptor-α (Ab-104) antibody.