• Catalog Number:

  E1A7068B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  This antiserum was produced against synthesized non-phosphopeptide derived from human Elk-1 around the phosphorylation site of Serine 383.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P19419

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000 IP: Various Dilution

• Specificity Sensitivity:

  Elk-1(Ab-383) Antibody detects endogenous levels of Elk-1 protein around Serine 383.

• References:

  Xing J, et al. (1996) Science. 273(5277): 959-963. Janknecht R, et al. (1993) EMBO J. 12(13): 5097-5104. Marais R, et al. (1993) Cell 73: 381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14: 4815-4824. Cavigelli M, et al. (1995) EMBO J. 14: 5957-5964. Whitmarsh A J, et al. (1995) Science. 269:403-407.

Immunohistochemical analysis of paraffin- embedded breast carcinoma. Left: Using Elk-1 (Ab-383) Antibody(#B7068); Right: Using the same antibody preincubated with synthesized peptide.

Western blot analysis of extracts from HeLa cells. Line1: Using Elk-1(Ab-383) Antibody (#B7068); Line2: Using the same antibody preincubated with synthesized peptide.