Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 1110 (P-V-YP-H-N).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
EGFR (Ab-1110) antibody detects endogenous levels of total EGFR protein.
References:
Kaisa Erjala, et,al. (2006) Clin. Cancer Res Jul 2006; 12: 4103-4111.
Judit Anido, et,al. (2003) Clin. Cancer Res; 9: 1274.
Julian Andreev, et,al. (2001) J. Biol. Chem; 276: 20130-20135.
Suil Kim, et,al. (2002) Am J Physiol Lung Cell Mol Physiol; 283: 67.
Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 10min), using EGFR (Ab-1110) antibody (Line 1 and 2) and EGFR (Phospho-Tyr1110) antibody (Line 3 and 4).