• Catalog Number:

  E1A7060B-3

• Amount:

  100ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human EGFR around the phosphorylation site of Serine1070.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  P00533

• Applications:

  WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

• Specificity Sensitivity:

  EGFR (Ab-1070) Antibody detects endogenous levels of total EGFR protein.

• References:

  Feinmesser RL,et al. (1999) J Biol Chem; 274(23): 16168-73. Gamou S,et al. (1995)FEBS Lett; 357(2): 161-4. Gamou S, et al. (1994)J Cell Physiol; 158(1): 151-9. Heisermann GJ, et al. (1988) J Biol Chem; 263(26): 13152-8.

Immunohistochemical analysis of paraffin- embedded breast carcinoma, using EGFR (Ab-1070) Antibody (#B7060). Left: Untreated; Right: Treated with synthesized peptide.

Western blot analysis of the extracts from SK-OV3 cells untreated or treated with EGF using EGFR(Ab-1070) Antibody ( #B7060) and EGFR(Phospho-Ser1070) Antibody (#11105): Line1: Using #B7060; Line2: Using #B7060 preincubated with synthesized peptide.