• Catalog Number:

  E1A7044B-1

• Amount:

  30ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  The antiserum was produced against synthesized non-phosphopeptide derived from human Chk2 around the phosphorylation site of serine 516 (Q-P-SP-T-S).

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human, Mouse, Rat

• SwissProtNo:

  O96017

• Applications:

  WB: 1:500~1:1000 ELISA: 1:20000

• Alter Name:

  CHEK2, CHK2, Cds1, Chk2, RAD53, kinase Chk2

• Specificity Sensitivity:

  Chk2 (Ab-516) antibody detects endogenous levels of total Chk2 protein.

• References:

  Shannon L. Gibson, et al. (2005) Cancer Res. 65: 10734-10741.  Jacques Huot, et al. (1996) Cancer Res. 56: 273-279.  Danita M. Bagaglio, et al. (1993) Cancer Res. 53: 2260-2264.  Kazuhiro Chida, et al. (1986) Cancer Res. 46: 1055-1062.

Western blot analysis of extracts from 293 cells untreated or treated with UV, using Chk2 (Ab-516) antibody (#B7043, Line1 and 2).