• Catalog Number:

  E1A7040B-2

• Amount:

  50ul

• Form of Antibody:

  Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage/Stability:

  Store at -20℃/1 year

• class="eg_cn_title"Immunogen:

  This antiserum was produced against synthesized non-phosphopeptide derived from human Chk1 around the phosphorylation site of Serine 280.

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Reactivity:

  Human

• SwissProtNo:

  O14757

• Applications:

  WB: 1:500~1:1000 ELISA: 1:10000

• Specificity Sensitivity:

  Chk1 (Ab-280) Antibody detects endogenous levels of total Chk1 protein.

• References:

  Conn CW, et al. (2004)Dev Cell; 7(2): 275-81. King FW, et al. (2004)Cell Cycle; 3(5): 634-7. Shtivelman E, et al. (2002) Curr Biol; 12(11): 919-24.

Western blot analysis of extracts from HeLa cells: Line1: Using Chk1 (Ab-280) Antibody( #B7040); Line2: The same antibody preincubated with synthesized peptide.