Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human CDC2 around the phosphorylation site of tyrosine 15 (G-T-YP-G-V).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse, Rat
SwissProtNo:
P06493
Applications:
WB: 1:500~1:1000 ELISA: 1:10000
Alter Name:
p34 protein kinase; Cyclin-dependent kinase 1; CDK1
Specificity Sensitivity:
CDC2 (Ab-15) antibody detects endogenous levels of total CDC2 protein.
References:
Jeremy P. H. Chow, et al. (2003)J. Biol. Chem; 278: 40815-40828.
NJ Wells, et al. (1999) J. Cell Sci; 112: 3361.
P Jin, et al. (1996) J. Cell Biol; 134: 963.
KM Pickham and DJ Donoghue (1994) Mol. Biol. Cell; 5: 587.
Western blot analysis of extracts from HepG2 cells, CDC2 (Ab-15) antibody (#Line 1 and 2) and CDC2 (phospho-Tyr15) antibody (Line 3).