Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:
Store at -20℃/1 year
class="eg_cn_title"Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1524(Y-P-SP-Q-E).
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Reactivity:
Human, Mouse
SwissProtNo:
P38398
Applications:
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Other Names:
BRCA1; Breast cancer type 1 susceptibility protein; familial breast/ovarian cancer gene 1
Specificity Sensitivity:
BRCA1 (Ab-1524) antibody detects endogenous levels of total BRCA1 protein.
References:
Miki Y., Science 266:66-71(1994).
Smith T.M., Genome Res. 6:1029-1049(1996).
Wilson C.A., Oncogene 14:1-16(1997).
Western blot analysis of extracts from COLO cells and HepG2 cells treated with serum (20%, 15mins) and 293 cells treated with UV (15mins), using BRCA1 (Ab-1524) antibody.